Composite

Part:BBa_K4968018:Design

Designed by: Shouye Zhu   Group: iGEM23_XJTLU-CHINA   (2023-10-09)


SmtA-CBM-sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 676
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 282
    Illegal BsaI.rc site found at 275


Source

The SmtA sequence is derived from NCBI. The sequence of the family 2 CBM from endoglucanase A of C.fimi was obtained from NCBI. The sfGFP sequence is derived from the Snapgene database. Linker between SmtA and CBM:GSGGAGGS. Linker between CBM and sfGFP: GGGSPTGG.

References

Blindauer, C.A. (2011) ‘Bacterial metallothioneins: Past, present, and questions for the future’, JBIC Journal of Biological Inorganic Chemistry, 16(7), pp. 1011–1024. doi:10.1007/s00775-011-0790-y.

Blindauer, C.A. et al. (2007) ‘Histidine ligands in bacterial metallothionein enhance cluster stability’, JBIC Journal of Biological Inorganic Chemistry, 12(3), pp. 393–405. doi:10.1007/s00775-006-0196-4.

Oliveira, N.M. et al. (2015) ‘Biofilm Formation as a response to ecological competition’, PLOS Biology, 13(7). doi:10.1371/journal.pbio.1002191.

Pédelacq, J.-D. et al. (2005) ‘Engineering and characterization of a superfolder green fluorescent protein’, Nature Biotechnology, 24(1), pp. 79–88. doi:10.1038/nbt1172.